MicroRNA-23b Promotes Avian Leukosis Virus Subgroup J (ALV-J) Replication by Targeting IRF1
نویسندگان
چکیده
Avian leukosis virus subgroup J (ALV-J) can cause several different leukemia-like proliferative diseases in the hemopoietic system of chickens. Here, we investigated the transcriptome profiles and miRNA expression profiles of ALV-J-infected and uninfected chicken spleens to identify the genes and miRNAs related to ALV-J invasion. In total, 252 genes and 167 miRNAs were differentially expressed in ALV-J-infected spleens compared to control uninfected spleens. miR-23b expression was up-regulated in ALV-J-infected spleens compared with the control spleens, and transcriptome analysis revealed that the expression of interferon regulatory factor 1 (IRF1) was down-regulated in ALV-J-infected spleens compared to uninfected spleens. A dual-luciferase reporter assay showed that IRF1 was a direct target of miR-23b. miR-23b overexpression significantly (P = 0.0022) decreased IRF1 mRNA levels and repressed IRF1-3'-UTR reporter activity. In vitro experiments revealed that miR-23b overexpression strengthened ALV-J replication, whereas miR-23b loss of function inhibited ALV-J replication. IRF1 overexpression inhibited ALV-J replication, and IRF1 knockdown enhanced ALV-J replication. Moreover, IRF1 overexpression significantly (P = 0.0014) increased IFN-β expression. In conclusion, these results suggested that miR-23b may play an important role in ALV-J replication by targeting IRF1.
منابع مشابه
Serologic profile of avian leukosis virus subgroup-J, Mycoplasma gallisepticum and Mycoplasma synoviae in broiler grandparent flocks of Iran.
Avian Leukosis Virus Subgroup-J (ALV-J). Mycoplasma gallisepticum (MG) and MycopJasma synviae (MS) are important pathogens in chickens that cause severe economical losses in poultry industry throughout the world. Seven broiler grandparent flocks of Iran (six broiler strains) were sampled randomly at the ages of 8-63 wk (100 samples from each flock) for antibody detection to ALV-J, MG, and MS ...
متن کاملDetection of avian leukosis virus subgroup J in albumen of commercial and native fowl eggs using RT-PCR in Fars province of Iran
The subgroup J of ALV (ALV-J) has emerged as an important pathogen of meat-type chickens since1989. This virus is responsible for economic losses due to both mortality and depressed performance inchickens. So, the objective of this study is the detection of ALV-J in the albumen of commercial and nativefowl eggs using RT-PCR. Three hundred and seventy egg albumens were randomly selected from dif...
متن کاملMetastatic cutaneous neuro- myofibroblastic sarcoma induced by avian leukosis virus subgroup J in a rooster (Gallus gallus domesticus)
An adult native cock (Gallus gallus domesticus) referred to the aviary clinic with multiple different sizes of round dermal nodules. The bird died few days later, and was then submitted for further evaluation. Macroscopic and microscopic examinations as well as a PCR test were done to identify type and cause of the tumor. In histopathological assessment of biopsy specimen, it consisted of inter...
متن کاملAberrant expression of liver microRNA in chickens infected with subgroup J avian leukosis virus.
Subgroup J avian leukosis virus (ALV-J) is an oncogenic retrovirus primarily causing myeloid leukosis (ML) in broilers. Although ALV is well under control in a few countries including the USA, poultry industry in many parts of the world continues suffering from serious economic loss due to sporadic or widespread ALV infection, especially ALV-J infection. ALV-J infection of chickens is reportedl...
متن کاملGADD45β, an anti-tumor gene, inhibits avian leukosis virus subgroup J replication in chickens
Avian leukosis virus subgroup J (ALV-J) is a retroviruses that induces neoplasia, hepatomegaly, immunosuppression and poor performance in chickens. The tumorigenic and pathogenic mechanisms of ALV-J remain a hot topic. To explore anti-tumor genes that promote resistance to ALV-J infection in chickens, we bred ALV-J resistant and susceptible chickens (F3 generation). RNA-sequencing (RNA-Seq) of ...
متن کامل